Archiral & Chiral Separation

Our in-house separation technology lab offers a full range of efficient and flexible techniques for the purification and separation of test compounds, intermediates, and batches for toxicological studies, using normal and reversed-phase HPLC/UPLC and supercritical fluid chromatography (SFC).

We offer special separation techniques for enantiomers and diastereomers for a variety of compound types, including small molecules, PROTACs, small peptides, macrocycles, and lipids. Our decades of experience lets us tailor-make the best solution for your separation problems.

Our routine separation services range from analytical determination of diastereomeric or enantiomeric purity to separation and purification of batches on a several-hundred grams scale.

Our daily routine work is purification with fast MS-guided automated preparative HPLC for all synthesis products on different scales. By centralizing purification in our separation lab, we ensure a time savings in synthesis, enhance the purification quality significantly, and reduce costs.

We also isolate unknown by-products, which are then characterized by our structure service unit. Very small amounts (e.g., a few milligrams) can be properly separated with special care by our well-trained team.

For our analytical services, we typically screen up to 200 different conditions (column, flow rate, media, etc.) to find the best conditions for your molecules. With this information, we can then provide enantiomeric excess studies (ee) for chiral product synthesis, such as in preparation for in vivo studies. Our SFC allows us to quickly screen and separate enantiomers or diastereomers up to 1 kg.

To ensure correct handling of compounds, each separated batch is lyophilized as a matter of routine. Our automated solvent systems mean we avoid direct contact with solvents, ensuring low solvent use and allowing for direct solvent removal and disposal.

All these services are supported by our fully digitized landscape by using a LIMS, ensuring data integrity and data security. The results can be sent directly to our customers via several interfaces with different systems.

We collaborate closely with all units at NUVISAN. For example, we separate samples from the microbiological chemistry laboratory to obtain pure metabolites, which are then characterized in our metabolite discovery platform. With our special SFC techniques, we are also collaborating closely with other laboratories, including physchem, and SFC can be used for special analytical measurements like experimental polar surface area (ePSA) services.