Chromatography and mass spectrometry

Proteins, peptides and new modalities - Experience the power of LC-MS for your protein analysis needs

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful technique for quantifying peptides, including insulin and its derivatives, and proteins like antibodies, antibody-drug-conjugates (ADC), biomarkers and recombinant proteins.  
We combine the following methods to achieve the highest possible sensitivity and accuracy in peptide and protein analysis: 

• Sample preparation suitable to your project needs – from direct digest to sophisticated immune capture using magnetic beads and a magnetic bead handling system
• Proteolytic digestion with suitable enzymes to provide unique surrogate peptides 
• Chromatographic separation using diverse 1D and 2D chromatography modes  
• Mass spectrometric detection using state-of-the-art instrumentation.  
  
  

In addition, we support your bioanalysis projects for other modalities: 

• Liposomes/LNPs 
• Excipients 
• Additional small molecule biomarker from different chemical classes 
• Metabolomic screening kits. 

Small molecules 

Method development of de novo assays or adaptation/transfer is our daily business. After validation in compliance with regulatory guidelines the methods can be used for sample analysis to support all phases of pharmaceutical development. 
We offer world-leading expertise and capabilities to develop the optimal assays for your target using high-end LC-MS/MS systems by experienced scientists.  
Our substantial method development and validation expertise encompasses very-high-sensitivity assays in the pg/mL range, chiral assays, peptide bioanalysis, dried-blood samples and microsampling, and ex-vivo plasma protein binding using the RED device.  
In addition, expertise with multiple processing techniques – including protein precipitation, liquid-liquid extraction, solid phase extraction, derivatization, and column switching – foster the development of the optimal method to meet each project’s requirements. 

Elemental analysis 

For elemental analysis, we  partner with ALS to provide advanced techniques for trace element investigations, supporting your research with a high degree of accuracy. 

Non-proprietary assays (clinical)

We offer many validated off-the-shelf non-proprietary assays to support DDI assessments and to include multicomponent cocktail type assays and assays to support PD parameters.