Metabolite discovery

Comprehensive metabolite discovery

Our metabolite discovery platform combines decades of expertise in both pharmaceuticals and crop protection to deliver comprehensive solutions for drug metabolite challenges. We support fermentation, biotransformation and synthesis of metabolites for pharmacological, DMPK, toxicological testing and analytical methods. Our platform efficiently identifies, screens, synthesises and characterises the metabolites essential to your research and development.

A visual representation of metabolite produced by the action of Streptomyces griseochromogenes on the HIF-PH inhibitor Molidustat

Metabolite discovery and analysis

Our platform provides end-to-end support for drug metabolite discovery, synthesis, purification and analysis.

Metabolite screening and synthesis

Screening: use of wild-type fungi, bacteria strains, human and bacterial CYPs in cells and isolated enzymes
Synthesis: late-stage diversification via chemical oxidations and glucuronidations
De novo synthesis: combining classical methods with modern techniques (photo- and electrochemistry).

Fermentation and scale-up

Fermentation platforms:
- 1–2 L Biostat B-DCU for optimisation
- 5–100 L steel fermenters for scale-up
Generation of drug metabolites in milligram to gram scale via biotransformation.

Purification and analytics

State-of-the-art chromatography (normal, reversed-phase, supercritical fluid)
Chiral separations and screening (up to 200 combinations)
Characterisation via NMR, HPLC, HRMS and UPLC-MS
Stability evaluations and physicochemical property measurement (logD, solubility, pKa).

DMPK support

Metabolite identification in biological matrices (in vitro, in vivo)
Metabolic stability, permeability and protein binding assessments