The design of suitable constructs is key for a successful structure determination for your target protein. Based on target experience, bioinformatic analyses, and limited proteolytic digest analyses, we identify a set of different-length construct variants suitable for addressing the aims of your program. Our protein science labs perform expression and purification tests to identify the optimal conditions for target protein production. Biophysical methods (including TSA and nanoDSF) may be used to identify optimal constructs, buffer composition, or early hit list compounds that stabilize the target protein and facilitate crystallization.
Our state-of-the-art crystallization platform, which enables fast and efficient screening to identify and optimize crystallization conditions, includes:
Regular bi-weekly access to third-generation high-brilliance synchrotron sources is complemented with our in-house automated crystal screening sample changer and two X-ray sources. Together, they support rapid and regular data collection. Our in-house X-ray platform includes:
Supported by our proprietary automated structure-solution pipeline, datasets are evaluated and structures are fully refined. Following a final internal quality control check, we deliver a structural biology data package containing a full set of industry-standard files.
Together with our DLS team, we complete the structure-determination activities by offering an in-depth binding mode analysis to support and accelerate structure-based compound optimization and to help enable full integration of structural learnings into your optimization programs.
Roehrig et al., Design and Preclinical Characterization Program toward Asundexian (BAY 2433334), an Oral Factor XIa Inhibitor for the Prevention and Treatment of Thromboembolic Disorders. JMedChem, 2023, 66 (17)