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Biochemical assays

From assay concept to lead optimisation 

Every screening project comes with its own unique challenges and therefore demands tailored strategies. At the core of each project is the design of robust assays, including biochemical assays. With decades of industry experience, our team excels in designing, developing, executing and interpreting biochemical assays across a wide range of targets, supporting high-throughput screening (HTS) campaigns as well as hit-to-lead and lead optimisation. 

Young scientists explains an automated screening system to another person.

Target classes and readouts 

Our scientists are highly experienced in developing biochemical assays for all stages of the screening tree, including primary, orthogonal, counter-screen, cross-species, selectivity and mode-of-action assays. We work with a wide range of targets and utilise various readout technologies and modalities. For instance, we have successfully developed HTS-compatible enzyme activity assays, including kinetic, end-point, single and coupled enzyme assays, as well as equilibrium-binding assays for protein–peptide, protein–protein, protein–DNA and protein–RNA interactions. 

Our expert scientists develop tailored biochemical assays across all stages of the screening process, using a wide range of targets and readout technologies. From HTS-compatible enzyme assays to equilibrium-binding assays for protein interactions, we deliver versatile solutions to meet your research needs. 

HTS assay development  

All biochemical assays are customised to align with your project goals, carefully considering your desired mode-of-action and helping ensure that in vitro conditions closely reflect physiological reality. With your project goals in mind, our scientists develop highly effective screening assays to help ensure a successful hit identification campaign. We take into account your desired mode-of-action—whether competitive, orthosteric or slow off-rate compounds—during assay development. To achieve in vitro conditions that best reflect physiological reality, we carefully characterise enzyme binding sites, substrates and co-factors. 

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Hit characterisation and mode-of-action assays  

Our multidisciplinary team works closely with you to characterise screening hits and refine lead-like compounds, maximising success and advancing your drug discovery with confidence. Our team of screeners, protein scientists, structural biologists and chemists collaborates closely with you to thoroughly characterise your screening hits. We leverage our combined knowledge and experience to identify and refine lead-like compounds that meet the specific profile you are targeting. Our goal is to deliver compounds that align seamlessly with your project requirements, maximising the potential for success and advancing your drug discovery process with confidence. 

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