Membrane protein platform

More than half of FDA-approved drugs target membrane proteins, which are difficult to study due to low expression levels and instability under in vitro conditions. G-protein-coupled receptors (GPCRs), the largest human membrane receptor family, make up one-third of prescribed medicines. Our membrane protein platform provides access to membrane proteins, including GPCRs, ion channels and transporters, for your drug discovery project.

Lab technician loads samples into the instrument for FSEC analysis

Optimising membrane protein purification and function

Our membrane protein experts specialise in purifying and characterising challenging membrane proteins, including ion channels, transporters and GPCRs. Our membrane protein platform features an efficient multistep production pipeline. In collaboration with you, we design constructs and clone genes into our in-house vector panel, utilising various expression systems such as baculovirus insect cells and Expi293 mammalian cells.

High-throughput small-scale expressions and purification are performed under target-specific conditions, with outcomes evaluated by fluorescence size-exclusion chromatography (FSEC) and nanoDSF. These results are optimised and scaled up for large-scale purifications. Iterative and final quality controls (SDS-PAGE, SEC, nanoDSF and mass spectrometry) help ensurethe production of highly pure, functional and monodisperse membrane proteins.

Overview of the protein production workflow

Membrane protein integration into nanoparticles

Membrane proteins exist in various conformational states that bind different ligands, such as antagonists, inverse agonists or agonists. Detergents can impact the equilibrium of these states. To provide a more native environment, we integrate purified targets into nanoparticles like nanodiscs, amphipoles or liposomes. We produce membrane proteins tailored to your needs, suitable for biophysics (e.g., CPM assay, SPR and nanoDSF), screening, biochemical assays, nanobody generation and structural biology. With our extensive experience, you can trust us to support your projects with high-quality membrane protein production.