Protein Detection Assays

Every novel therapeutic target will lead to new challenges in monitoring relevant downstream effects on proteins. At NUVISAN, we can help you develop novel assays on even unprecedented target proteins or modifications of proteins. In addition to traditional techniques (e.g., Western blot, ELISA, Meso Scale Discovery technology), we have experience with more sophisticated techniques, such as the Simple Western^TM system, which is an automated, quantitative, and higher-throughput approach to protein detection using capillary-based separation of protein targets by size.

We also use immunohistochemistry (IHC) and spatial profiling to detect different target proteins and/or modifications in different tissues to answer biomarker-relevant questions. Please inquire about your specific needs.

Exemplary services we offer:

• Western blot
• Dot-blot
• Simple Western (capillary-based separation of protein using the Simple Western^TM system)
• In-cell Western (ICW)
• FACS
• ELISA, MSD
• G-Lisa, HTRF assays, peptide arrays
• Global proteomic studies (e.g., SILAC, iTRAQ multiplexing)
• High-content assays
• Immunohistochemistry (IHC)
• Spatial profiling

In-cell Western assay for the detection of so far unprecedented AHNAK protein methylation by SMYD2. Each row represents a different SMYD2 inhibitor tested in increasing concentrations ranging from 39 nM to 50 μM. Example taken from Eggert, et al., J Med Chem (2016).