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Thermal stability assays 

Monitoring protein stability and binding interactions 

Nuvisan boasts a long history of expertise in differential scanning fluorimetry (DSF), also known as thermal shift assays (TSA) or ThermoFluor. This technique monitors temperature-mediated protein unfolding and detects changes in the thermal stability of a target upon ligand binding, effectively differentiating between binders and non-binders. Our skilled scientists can support you in designing and conducting DSF experiments to identify and characterise potential binders, enabling valuable insights for your research and development projects. 

Scientist verifying DSF data in the laboratory 

High-throughput thermal stability assays and protein stability analysis 

We leverage our advanced DSF setup, featuring Thermo Fisher instruments (QuantStudioand ViiA7 systems) and plate automation, to efficiently screen libraries of up to 350,000 compounds. Our in-house co-developed software (Genedata) enables precise hit analysis. We support your projects beyond hit validation, applying DSF for mode-of-action studies to reveal critical insights such as the importance of co-factor binding for specific compound sets. We also evaluate the effect of different buffer conditions on protein stability and guide structural biology by assessing the folding behavior of various protein constructs. Additionally, our DSF expertise extends to fragment screens, aiding in the development of highly valuable tool compounds. For thermal shift assays with membrane proteins, where common dyes are unsuitable, we use a thiol-reactive fluorescent dye, CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin). Our CPM assays enable successful study of substrate-induced thermal stabilisation of GPCRs and SLC transporters, to provide you with critical insights into their functionality.  

Label-free protein stability with nanoDSF 

Our biophysicists employ nano-differential scanning fluorimetry (nanoDSF), a label-free method for measuring protein stability to study the effects of buffers, co-factors and ligands on protein stability with high sensitivity. While the throughput of nanoDSF is lower than that of DSF, its low protein requirements make it one of our preferred methods for guiding the optimisation of protein purification at all stages, including the production of membrane proteins. Our expert team is ready to support you in achieving precise and efficient protein stability assessments tailored to your specific research needs. 

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