Technology platforms

Empowering drug discovery with advanced technology

Our expertise in disease and target biology is enhanced by state-of-the-art technology platforms, supporting your drug discovery projects across all indications and modalities. We monitor functional readouts, such as cellular phenotypic drug responses, using high-content assays, Meso Scale Discovery, precision protein analysis and multiparametric flow cytometry (FACS). Our next-generation sequencing and single-cell sequencing services provide mode-of-action analysis at excellent resolutions.

Multicolor flow cytometry

Our multicolour flow cytometry services are essential for identifying new drug targets, screening potential drug candidates and analysing drug effects on specific cell populations. We offer comprehensive panels for immunoprofiling with established and validated assays for all major mouse and human immune cell populations. Our advanced flow cytometry devices include Cytek Aurora (full-spectrum technology), BD Canto II, Sartorius Intellicyt iQueScreener PLUS and Sony MA900 cell sorter. Selected applications include:

Immuno-oncology
Biomarker identification and validation
Target identification and validation
Compound mode-of-action and efficacy
Immunoprofiling
Immunophenotyping of all immune cell populations, including T, B, and NK cells, regulatory T cells, naïve and activated T cells, memory and effector T cells, naïve and memory B cells, non-classical and classical monocytes, macrophages and dendritic cells, with room for additional markers and dyes for tailored scientific investigations
Antibody screening
Cell sorting for subsequent single-cell sequencing.

Precision protein analysis for mode-of-action insights

Our protein detection assay services focus on detecting and quantifying protein expression, modification and regulation. This is crucial for addressing key project questions related to mode-of-action, pharmacodynamic biomarkers and the efficacy of lead candidates. We offer a range of assay formats tailored to meet the specific needs of each of your projects and develop novel assays for unprecedented target proteins or their modifications. We utilise both traditional techniques, such as western blot and ELISA, and advanced methods like the Simple Western system, which provides an automated, quantitative and high-throughput approach to protein detection through capillary-based separation. Our services include:

Western blot
Dot-blot
Simple Western (capillary-based separation of protein using the Simple Western system)
In-cell Western (ICW)
ELISA, MSD
G-Lisa, HTRF assays, peptide arrays
FACS
Global proteomic studies (e.g., SILAC, iTRAQ multiplexing)
High-content assays
Immunohistochemistry (IHC)
Spatial profiling.

Tailored next-generation sequencing services

Our next-generation sequencing (NGS) services are customised to meet your specific needs, including experimental design, professional lab execution, in-house NGS sequencing and tailored bioinformatics analysis — all conducted at our Berlin site. We utilise state-of-the-art NGS technology to identify novel targets and analyse the mode-of-action of compounds with excellent resolution. Our 10x Genomics single-cell sequencing is particularly effective for measuring exploratory biomarkers in preclinical and clinical studies. Our services include:

DNA and RNA isolation services
Transcriptome sequencing (RNA-seq)
Genome sequencing
Exome sequencing
Ribo-Seq
SLAM-Seq
High-throughput (DRUG-) seq
Epigenetic profiling (ChiP-seq, methylome-seq, ATAC-seq)
Amplicon and panel sequencing
Barcode-seq (for CRISPR screening, AAV and LNP tissue distribution)
10x Genomics single-cell sequencing (scRNA-seq, scATAC-seq, T- and B-cell receptor profiling, CITE-seq, perturbation-seq, custom scAmplicon-seq).

Flyer: degrader platform

Nuvisan provides a fully integrated protein degradation platform encompassing comprehensive PROTAC® and molecular glue drug discovery services.

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Biomarker discovery

Advance precision medicine with Nuvisan’s expertise in biomarker discovery.

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